Expression pattern of a novel hyaluronidase during Xenopus embryogenesis
نویسندگان
چکیده
منابع مشابه
Expression pattern of a novel hyaluronidase during Xenopus embryogenesis
We have isolated a cDNA encoding a novel hyaluronidase, which is expressed during embryogenesis. The encoded protein was expressed as a fusion polypeptide with glutathione S-transferase, and the affinity-purified fusion protein was shown to possess hyaluronidase activity with a pH optimum about pH 4.0. The expression of the XEH1 gene was analysed by in situ hybridization, and was first apparent...
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We have identified a member of the insulin receptor (InsR)/insulin-like growth factor-1 receptor (IGF-1R) family. The Xenopus insulin receptor (Xe-InsR) is present as a maternal 6.6 kb transcript. Northern blot analysis reveals the presence of this transcript until the mid-blastula transition (MBT), when levels decrease. At neurulation, two distinct transcripts of 6.6 and 7.7 kb are detected, b...
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We have cloned and characterized a second member of the Xenopus CAP (cyclase associated protein) gene family. xCAP2 demonstrates greater restriction of expression than its homolog, xCAP1, and is differentially expressed throughout early embryogenesis. Although present as a maternal transcript, CAP2 comes to be expressed in the anterior-most mesoderm/endoderm during late gastrulation, in paraxia...
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A Hyal2-type hyaluronidase of Xenopus laevis (Xhyal2) was characterized by molecular cloning, biochemical analysis and ectopic overexpression in embryos. When expressed in Xenopus oocytes, Xhyal2 exists as a soluble protein in the extracellular space and in intercellular compartments as well as being attached to the cell surface through a glycosyl-phosphatidyl-inositol anchor. This enzyme speci...
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We have identified a cDNA encoding a novel Xenopus winged helix transcription factor termed as XFD-11 (Xenopus fork head domain). The DNA binding domain is most closely related to those of human or murine FREAC-3 (FKHL7/MF-1/FKH-1) proteins. The XFD-11 gene is activated at late blastula/early gastrula and transcription proceeds throughout embryogenesis. Early expression is found in ventral and ...
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ژورنال
عنوان ژورنال: Mechanisms of Development
سال: 1999
ISSN: 0925-4773
DOI: 10.1016/s0925-4773(99)00019-2